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2. DETERMINATION
OF GLOMERULAR FILTRATION RATE FROM PLASMA CLEARANCE WITH Tc99m-DTPA
BY SINGLE; TWO AND MULTI-SAMPLE METHODS.
Kazuo Itoh,
Satoshi Tsushima, Eriko Tsukamoto and Nagara Tamaki, ·
Departments of Radiology, Internal Medicine and Nuclear
Medicine,
JR Sapporo General Hospital and Hokkaido University School of
Medicine, Sapporo,JAPAN.
itohka51@ra2.so-net.ne.jp
Background.The
plasma sample method following a single injection of radioactive
markers has been proved to be simple and accurate for the determination
of glomerular filtration rate (GFR) in clinical practice. The
aim of this study was to assess clinical accuracy of single-,
two-and multi-sample methods.
Patients
and Methods. The study was performed on 50 patients with
various degrees of renal dysfunction (29 males and 21 females;
aged 27-90 years). As a reference the true GFR was determined
by means of the two compartment model curve fitting 10 plasma
following a single-injection of Tc99m-DTPA. The GFR was estimated
by the three methods of single-, two and multi-sample between
75 and 300 min after the injection. The GFR by two and multi-sample
methods was determinated with the slope and intercept algorithm
and its overestimation was corrected by Brochner-Mortensen's
formula.
Results.
In all patients with a GFR between 12 and 169 ,
accuracy for the determination of GFR was identical in both
the single-and five-sample methods. In 46 patients with a GFR
higher than 30 ,
the single-sample method exhibited the lowest standard error
of mean of difference in GFR. In 38 patients with a GFR lower
than 120 ,
the two and five sample methods were more accurate than the
single-sample one. The best combination of sampling time for
the two samples was 120 min and 240 min after the injection.
Conclusion.
Accuracy of each plasma sample method depends on the preserved
GFR. As a matter of practice, the single-sample method is the
first choice for a patient who is expected to have a GFR higher
than 30 .
The two-sample method is indicated only in a patient with severe
renal failure with a GFE lower than 30 .
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